anti ifnγ mab  (Bio X Cell)

Journal: iScience

Article Title: A recombinant Cedar virus preclinical model that recapitulates neurological features of henipavirus disease

doi: 10.1016/j.isci.2025.113571

Figure Lengend Snippet: Peripheral neutralization of IFN-γ results in enhanced in vivo transcription of rCedV-NiV-Luc and rCedV-HeV-Luc (A–C) Albino IFNAR-KO mice were infected with 10 7 PFU of rCedV-NiV-Luc or rCedV-HeV-Luc. Mice were administered anti-IFN-γ (200 μg XMG1.2 per dose) every 3–4 days, starting on day 0. Animals were analyzed by BLI on the indicated days p.i. Whole body and head-only BLI values for IFN-γ-treated albino IFNAR-KO animals (closed symbols, solid lines) infected with (B) rCedV-NiV-Luc or (C) rCedV-HeV-Luc ( n = 3–6 animals per group; error bars are SD). Data from untreated animals in are shown for comparison (open symbols, dashed lines). (D) Quantification of isotypes of NiV G-specific antibodies in mice infected with rCedV-NiV-Luc vs. mock-infected controls. (E) In vitro neutralization analysis of sera from animals infected with rCedV-NiV-Luc or rCedV-HeV-Luc, compared to mock-infected animals. ∗ p < 0.05. Sera were collected at experiment endpoint ( n = 3–6 animals per group; error bars are SD).

Article Snippet: Albino IFNAR-KO mice were administered 200 μg of anti-mouse IFNγ mAb (clone XMG1.2, BioXCell, cat #BE0055) via i.p. injection on days 0, 3, 6, 9, 13, 17, 21 post-infection (p.i.).

Techniques: Neutralization, In Vivo, Infection, Comparison, In Vitro